Menstruation is a physiological phenomenon among females. However, its socio-cultural and psychological interpretations vary in different societies. Whereas in Pakistan, discussion about it is a taboo. In this context,  this study determines the sources of menstruation hygiene awareness among adolescent girls in urban as well as rural areas of Sindh province, Pakistan. The present study applies a quantitative cross-sectional survey; it is coupled up with the close-ended questionnaire, which was distributed among the 100 adolescent girls of schools and colleges of one of the districts of Sindh province, Pakistan. The sample was selected by applying a random sampling technique. The findings revealed that most of the girls in Sindh Pakistan do not understand the basis of menstrual blood. A low proportion who were aware of menstruation, they got knowledge from their mothers. Further, most of the respondents were afraid at the first time bleeding of menstruation, and half of them told that they avoided taking a bath during periods. Finally, this study concludes that mothers are the primary information sources for girls about menstruation and the majority of the girl students are less aware of menstruation hygiene and the related practices.